Journal: Radiation research

Article Title: Topical Esomeprazole Mitigates Radiation-Induced Dermal Inflammation and Fibrosis

doi: 10.1667/RR15398.1

Figure Lengend Snippet: Western blot analysis of erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO1) proteins in homogenates derived from irradiated EpidermFT tissues. Nuclear and cytoplasmic proteins were fractionated and Nrf2 was probed in the nuclear fraction using rabbit anti-Nrf2 antibody. The housekeeping gene histone H3 was probed using rabbit anti-histone H3 antibody. HO1 was probed in the cytoplasmic fraction using rabbit anti-HO1 and GAPDH was detected using mouse anti-GAPDH antibody. The secondary antibody used was anti-rabbit monoclonal or anti-mouse monoclonal. The data show that dermaprazole upregulated the protein expression of Nrf2 and HO1.

Article Snippet: A three-dimensional (3D) human skin model (EpiDermFT, MatTek Corp., Ashland, MA) was used to validate the ability of formulated esomeprazole to engage biological targets.

Techniques: Western Blot, Derivative Assay, Irradiation, Expressing

Journal: Radiation research

Article Title: Topical Esomeprazole Mitigates Radiation-Induced Dermal Inflammation and Fibrosis

doi: 10.1667/RR15398.1

Figure Lengend Snippet: Western blot analysis of heme oxygenase 1 (HO1) protein in homogenates derived from nonirradiated EpidermFT tissue. Dermaprazole (1–2%) was applied on the tissue topically and the viable EpidermFT tissue was incubated at 37°C/5%CO2 for 24 h. HO1 was probed using rabbit anti-HO1 and GAPDH was detected using mouse anti-GAPDH antibody. The secondary antibody used was anti-rabbit monoclonal. The data show that dermaprazole upregulated HO1 expression in the absence of radiation.

Article Snippet: A three-dimensional (3D) human skin model (EpiDermFT, MatTek Corp., Ashland, MA) was used to validate the ability of formulated esomeprazole to engage biological targets.

Techniques: Western Blot, Derivative Assay, Incubation, Expressing

Journal: Radiation research

Article Title: Topical Esomeprazole Mitigates Radiation-Induced Dermal Inflammation and Fibrosis

doi: 10.1667/RR15398.1

Figure Lengend Snippet: Gene expression profiling of the transcription factor erythroid 2-related factor 2 (Nrf2) and the antioxidant enzyme heme oxygenase 1 (HO1) in irradiated EpidermFT tissue homogenates from a 3D human skin model. The EpidermFT was exposed to various strengths of dermaprazole, vehicle cream or the steroid hydrocortisone (1%) for 24 h. Fold change normalized to the vehicle control is shown. Data are from duplicate experiments. *P < 0.05 compared to the expression of Nrf2 in the vehicle and +P < 0.05 compared to the expression of HO1 in the vehicle group.

Article Snippet: A three-dimensional (3D) human skin model (EpiDermFT, MatTek Corp., Ashland, MA) was used to validate the ability of formulated esomeprazole to engage biological targets.

Techniques: Gene Expression, Irradiation, Cream, Control, Expressing